201 THE RELATIONSHIP OF AMNIOTIC FLUID DEHYDROISOANDROSTERONE SULFATE (DS) AND CORTISOL (F) CONCENTRATIONS WITH LECITHIN TO SPHINGOMYELIN (L/S) RATIOS DURING HUMAN CESTATION
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چکیده
منابع مشابه
Simple, rapid determination of lecithin and sphingomyelin in amniotic fluid.
We describe a rapid and very sensitive modified determination of lecithin and sphingomyelin in amniotic fluid. It requires 1.5 ml of amniotic fluid and can be done in less than 40 mm. Modifications include “Instant Thin Layer Chromatography” plates (Gelman) with a punched disc system of sample application, as well as elimination of the acetone wash steps, and substitution of Rhodamine B dye for...
متن کاملNew method for determining lecithin and sphingomyelin in amniotic fluid.
We describe a new method of complete analysis for lecithin, lysolecithin, and sphingomyelin in amniotic fluid. The analysis is based on alkaline hydrolysis of the lecithins and the enzymic hydrolysis of sphingomyelin. The choline formed in each instance is enzymically phosphorylated with [gamma-32P]ATP to yield [gamma-32P]phosphorylcholine, which is isolated by anion-exchange chromatography and...
متن کاملthe influence of corpus luteum on follicular fluid composition from different size follicles and their relationship to serum concentrations in sanjabi ewes
the aim of the present study was to determine the in?uence of presence or absence of corpus luteum (cl) on hormonal and metabolites composition of follicular ?uid (ff) harvested from different sized follicles and its relationship with blood serum concentrations in sanjabi ewes. ovaries and blood samples were collected from 60 clinically healthy adult ewes (sanjabi breed) 1–3 years of age in dio...
A modified procedure for evaluation of the lecithin-sphingomyelin ratio in amniotic fluid.
We describe several modifications of the procedure originated by Gluck et al. [Amer. J. Obstet. Gynecol. 109, 440 (1971)] for evaluation of amniotic fluid lecithin/sphingomyelin (L/S) ratios. The acetone-precipitation step is eliminated, resulting in a faster extraction procedure and higher concentrations of phospholipids. For faster thin-layer chromatography, we use silica gel-impregnated shee...
متن کاملAutomated enzymatic measurement of lecithin, sphingomyelin, and phosphatidylglycerol in amniotic fluid.
We describe methods for automated enzymatic measurement of lecithin, sphingomyelin, and phosphatidylglycerol in amniotic fluid. Phospholipase C (EC 3.1.4.3) and sphingomyelin phosphodiesterase (EC 3.1.4.12) are reacted with lecithin and sphingomyelin, respectively, to liberate phosphocholine. Phosphocholine is then reacted with alkaline phosphatase, choline oxidase, peroxidase, and 4-aminoantip...
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ژورنال
عنوان ژورنال: Pediatric Research
سال: 1978
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-197804001-00206